WebIncubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) in 100 ml 1X PBS. Store at 4°C. ... Anti-rat (#4416, #4418) B. Fixation. NOTE: If live cell staining is desired, proceed to Immunostaining (Section D). Please refer to the product webpage and product-specific protocol to determine whether it is compatible with live cell staining. WebRequest Bulk Quote. Description. Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell …
Propidium Iodide (PI)/RNase Staining Solution
WebBackground. Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). … WebDescription. Stain Buffer (FBS) can be used for the immunofluorescent staining of single-cell suspensions prepared from either lymphoid tissues, bone marrow, peripheral blood, … round 3.151 to the nearest hundredth
What are the possible reasons for this cell cycle results?
WebCell Staining Buffer Recipe. Recipe. 1.Dissolve 8g of NaCl, 0.2g of KCl, 1.44g of Na 2 HPO 4, and 0.24g of KH 2 PO 4 in 800ml distilled H 2 O. 2.Add 20 ml of heat inactivated FBS. 3.Add 0.9 grams of sodium azide. 4.Adjust pH to 7.4 with HCl. 5.Adjust volume to 1L with additional distilled H 2 O. 6.Sterilize by filtration. WebCells from Jurkat cell line (Human T-cell leukemia; ATCC TIB-152) were fixed with 1% paraformaldehyde (methanol free) and stored in 70% ethanol at -20°C. Cells were stained with 0.5 ml of PI/RNase Staining Buffer (Cat. No. 550825) for 15 minutes at room temperature and analyzed by flow cytometry. Show More. Protocol Library. Scientific … WebSep 8, 2016 · The Big Picture: DNA Stains for Cell Cycle Analysis. Staining for DNA content is the simplest way to divide your cells into G 0 /G 1, S, and G 2 /M phases. The … round 315.857 to the nearest hundredth