Dsred expression cassette
WebJul 2, 2012 · We confirmed the expression of the DsRed cassette in syncytia by growing seedlings from homozygous lines on agar and infection with H. schachtii larvae. As expected, in these lines, the Pdf2.1 promoter directed DsRed expression not only in seeds but also in syncytia (Figure 5 A and B). WebTo clarify this we developed a reliable assay to quantify the in vitro fusion frequency of cells using a fluorescence double reporter vector (pFDR) containing a LoxP-flanked HcRed/DsRed expression cassette followed by an EGFP expression cassette.
Dsred expression cassette
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WebTwo different SPG‐cell‐specific Gal4 drivers were used to direct expression, the previously described SNG‐Gal4 12 and our Mdr65‐Gal4 driver. 23 The ATP binding cassette (ABC) transporter Mdr65 has been shown to be specifically expressed in the SPG cells of the BBB, and Mdr65‐Gal4 reflects this expression. 23 , 29 , 30 Control flies ... WebJun 1, 2003 · DsRed-Express expression was detected as orange fluorescence with the GFP2 filter (not shown), because this filter allows detection of light above 510 nm and the stereomicroscope is equipped with a separate channel for the excitation light (no beam splitter needed).
WebOne important role for the expression of DMP1 in the nucleus of preosteoblasts is the up‐regulation of osteoblast﹕pecific genes such as osteocalcin and alkaline phosphatase. ... a 2﹌b fragment of DMP1 promoter, which was inserted upstream of the GFP and DsRed reporter cassette, was integrated into the human ROSA locus to generate double ... WebJun 21, 2024 · The control targeting vector carrying an expression cassette of FRT3-LC-IRES-HC-DsRed-IRES-FRT was constructed by Genscript. LC and HC represented the rituximab light chain cDNA and heavy chain ...
WebpIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) between the MCS and red fluorescent protein DsRed-Express2 coding region. This permits both the gene of … WebSep 25, 2015 · High and uniform DsRed expression was observed at the single cell and proliferating callus stages, in somatic embryos and within organs of whole in vitro and greenhouse-grown plants in a manner similar to GFP. ... The 35S-promoter-driven DsRed cassette was excised from p900 as an Xba1/Pst1 fragment and cloned into …
WebDownload scientific diagram DsRed-expressing cassette insertion in the human artificial chromosome (HAC) and EGFP expression from the HAC using dual recombinase …
WebApr 24, 2024 · Second, the co-expression of GFP and DsRed from pGB-R-GFP-DsRed and pGB-R-DsRed-GFP multi-cassette vectors was studied. It was found that the … latin america kise kahate hainWebTo overcome the slow maturation of DsRed, we used random and directed mutagenesis to create variants that mature 10–15 times faster than the wild-type protein.An asparagine … latin anniWebMay 20, 2005 · An expression cassette comprises targeting sites flanking a Cre fusion gene and a Cre coding sequence interrupted by an intron. ... C-D; Over-expression of Cre/Dsred fusion protein is toxic for the cells. E-G; Controlled expression solves toxicity associated to over-expression of Cre/DsRed. H-J; Red fluorescence disappears by time … latin animal taxonmyWebAug 1, 2024 · The first successful report of DsRed protein in plants was in transgenic tobacco, where its transient expression and stable transformation did not lead to … latin aoristoWebMay 28, 2024 · Construction of the pFS4 vector.a Three expression cassettes in the T-DNA. Pollen lethal gene cassette was about 4.7 Kb; the DsRed fluorescent bioreporter gene cassette was about 3 Kb; and a rice female fertility gene cassette was about 3 Kb.b Identification of pFS4 by restriction digest. M1-DL15000 marker; lane 1, pFS4 plasmid; … latin asistWebHygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors … latin amerika eksi sozlukWebThe Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged. The schematic below shows the three types of rearrangements ... latin amerika historie