site stats

Dsred expression cassette

WebJan 16, 2007 · In all cases, DsRed expression levels in the retina were higher than those observed when each cell-type-specific promoter was used to directly drive expression of DsRed. ... The mir30 expression cassette has the hairpin stem composed of siRNA sense and antisense strands (22 nt each), a loop derived from human miR30 (19 nt), and 125 … WebApr 22, 2015 · After Cre-mediated recombination the loxP-flanked HcRed/DsRed cassette is excised resulting in a stable EGFP expression and fluorescence (Fig. 1a). In Fig. 1 a, pFDR-expressing HS578T and MDA-MB-435 breast cancer cells were treated with 2 µM recombinant HTN Cre-fusion protein [ 30 ], which contains a TAT-leader domain enabling …

Transient protein expression in tobacco BY-2 plant cell …

WebNov 14, 2008 · in eukaryotic cells (4).The entire DsRed-Express2 expression cassette in pDsRed-Express2 is supported by a pUC19 backbone, which contains a high-copy number origin of replication and an ampicillin resistance gene (Amp. r) for propagation and selection in . E. coli. Use. pDsRed-Express2 is primarily intended to serve as a source of DsRed ... WebApr 9, 2024 · The hisB expression cassette of 1.6 kb was amplified from the genomic DNA of the N402 strain using the specific primer pair AnhisB-F/AnhisB-R (Table 2). ... Heterologous expression of the DsRed reporter gene in A. niger. A The schematic map of the binary vector pEX2E. The recognition sites of specific restriction enzymes were … latin amas vote https://salermoinsuranceagency.com

Genes Free Full-Text mCherry-Labeled Verticillium dahliae Could …

WebSeven days later, the cells were initially sorted based on DsRed2 expression; 6 clones that were GFP negative but DsRed positive were isolated, and 4 clones were confirmed by Southern blot ... WebAug 6, 2024 · One plasmid (PJET-DsRed) contained a red fluorescent protein DsRed expression cassette and homologous left (1,356 bp) and right (1,331 bp) arms, while the other plasmid (PJET-DsRed-MaSp1) … WebJun 1, 2003 · Abstract. The recently reported red fluorescent protein DsRed from the reef coral Discosoma sp. represents a new marker that has been codon-optimized for high … latin america jokes

Evaluation of red fluorescent protein (DsRed) as alternative visual ...

Category:Evaluation of red fluorescent protein (DsRed) as alternative visual ...

Tags:Dsred expression cassette

Dsred expression cassette

DsRed-expressing cassette insertion in the human

WebJul 2, 2012 · We confirmed the expression of the DsRed cassette in syncytia by growing seedlings from homozygous lines on agar and infection with H. schachtii larvae. As expected, in these lines, the Pdf2.1 promoter directed DsRed expression not only in seeds but also in syncytia (Figure 5 A and B). WebTo clarify this we developed a reliable assay to quantify the in vitro fusion frequency of cells using a fluorescence double reporter vector (pFDR) containing a LoxP-flanked HcRed/DsRed expression cassette followed by an EGFP expression cassette.

Dsred expression cassette

Did you know?

WebTwo different SPG‐cell‐specific Gal4 drivers were used to direct expression, the previously described SNG‐Gal4 12 and our Mdr65‐Gal4 driver. 23 The ATP binding cassette (ABC) transporter Mdr65 has been shown to be specifically expressed in the SPG cells of the BBB, and Mdr65‐Gal4 reflects this expression. 23 , 29 , 30 Control flies ... WebJun 1, 2003 · DsRed-Express expression was detected as orange fluorescence with the GFP2 filter (not shown), because this filter allows detection of light above 510 nm and the stereomicroscope is equipped with a separate channel for the excitation light (no beam splitter needed).

WebOne important role for the expression of DMP1 in the nucleus of preosteoblasts is the up‐regulation of osteoblast﹕pecific genes such as osteocalcin and alkaline phosphatase. ... a 2﹌b fragment of DMP1 promoter, which was inserted upstream of the GFP and DsRed reporter cassette, was integrated into the human ROSA locus to generate double ... WebJun 21, 2024 · The control targeting vector carrying an expression cassette of FRT3-LC-IRES-HC-DsRed-IRES-FRT was constructed by Genscript. LC and HC represented the rituximab light chain cDNA and heavy chain ...

WebpIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) between the MCS and red fluorescent protein DsRed-Express2 coding region. This permits both the gene of … WebSep 25, 2015 · High and uniform DsRed expression was observed at the single cell and proliferating callus stages, in somatic embryos and within organs of whole in vitro and greenhouse-grown plants in a manner similar to GFP. ... The 35S-promoter-driven DsRed cassette was excised from p900 as an Xba1/Pst1 fragment and cloned into …

WebDownload scientific diagram DsRed-expressing cassette insertion in the human artificial chromosome (HAC) and EGFP expression from the HAC using dual recombinase …

WebApr 24, 2024 · Second, the co-expression of GFP and DsRed from pGB-R-GFP-DsRed and pGB-R-DsRed-GFP multi-cassette vectors was studied. It was found that the … latin america kise kahate hainWebTo overcome the slow maturation of DsRed, we used random and directed mutagenesis to create variants that mature 10–15 times faster than the wild-type protein.An asparagine … latin anniWebMay 20, 2005 · An expression cassette comprises targeting sites flanking a Cre fusion gene and a Cre coding sequence interrupted by an intron. ... C-D; Over-expression of Cre/Dsred fusion protein is toxic for the cells. E-G; Controlled expression solves toxicity associated to over-expression of Cre/DsRed. H-J; Red fluorescence disappears by time … latin animal taxonmyWebAug 1, 2024 · The first successful report of DsRed protein in plants was in transgenic tobacco, where its transient expression and stable transformation did not lead to … latin aoristoWebMay 28, 2024 · Construction of the pFS4 vector.a Three expression cassettes in the T-DNA. Pollen lethal gene cassette was about 4.7 Kb; the DsRed fluorescent bioreporter gene cassette was about 3 Kb; and a rice female fertility gene cassette was about 3 Kb.b Identification of pFS4 by restriction digest. M1-DL15000 marker; lane 1, pFS4 plasmid; … latin asistWebHygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors … latin amerika eksi sozlukWebThe Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged. The schematic below shows the three types of rearrangements ... latin amerika historie