site stats

Pef6/myc-his

WebpEF6/myc-His C; Vector Database. Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … WebApr 27, 2007 · The pEF6- lacZ expression construct was obtained from Invitrogen. Cell Culture and Transfection —DT40 chicken B cells were grown in RPMI supplemented with 10% fetal bovine serum, 1% chicken serum, 100 units/ml penicillin, 100 μg/ml streptomycin, 50 μ m mercaptoethanol, and 20 m m GlutaMAX.

Addgene: Vector Database - pEF6/myc-His C

WebpcDNA-HA and pEF6-MYC:His 6 (Invitrogen). Oligo- ... Basel, Switzerland) or anti-MYC antibodies (mouse IgG1, clone 9E10, SCB, Santa Cruz Biotechnology, CA, US) for 2 hours at 4°C with agitation ... http://www.biovector.net/product/44170.html four bad boys and me cast https://salermoinsuranceagency.com

HCV: Transfer of Splenocytes to Study Cell-mediated Immune ... - Medscape

WebFeb 22, 2024 · Chinese hamster ovary cells were transiently transfected with the recombinant pEF6/Myc-His vector containing the core/E1/E2 insert. Transfection was performed by 2 electroporation shocks at... WebDec 22, 2016 · We then analyzed the expression of v-AKT and c-AKT in HEK293T cells that were transfected with the FeLV–AKT F1344 provirus or Myc-tagged c-AKT1, v-AKT clone 1, clone 2, or clone 3 cloned into the pEF6/Myc–His expression vector (Life Technologies). WebThe vector pEF6-myc-HisB, which has an EF promotor, was chosen because of its ability to drive strong expression of the protein of interest in mammalian cells. The readers should … four bad boys and me summary

pEF6/V5-His TOPO™ TA Expression Kit

Category:Pef6 Myc His Vector Thermo Fisher Bioz

Tags:Pef6/myc-his

Pef6/myc-his

pEF6 V5-His A Sequence and Map - SnapGene

WebComments for pEF6/Myc-His A 5798 nucleotides EF-1α promoter: bases 386-1545 T7 promoter/priming site: bases 1546-1565 Multiple cloning site: bases 1591-1681 myc … http://www.biofeng.com/plus/view.php?aid=13720

Pef6/myc-his

Did you know?

WebMar 9, 2015 · pEF4/myc-His vectors are shipped on wet ice. Upon receipt, store vectors at -20°C. Kit Contents All vectors are supplied as detailed below. Store the vectors at –20°C. … WebDec 4, 2024 · The pEF6/Myc-His vectors harbouring FL-cPLA 2 ɛ and its mutants or insert-free pEF6/Myc-His vector were introduced into HEK293 cells using Lipofectamine 2000. After 48 h of transfection, cells were harvested and sonicated twice each for 5 s in 20 mM Tris–HCl (pH 7.4).

WebSep 25, 2024 · pEF6/Myc-His vector harboring C-terminally FLAG-tagged human PLAAT-2 cDNA [ 12] ( see Note 1 ). 8. Branson Sonifier model 250 equipped with a tapered microtip (end diameter of 1/8 inch) (Branson, Danbury, CT, USA). 9. Buffer C: 20 mM Tris-HCl (pH 7.4). 10. Buffer D: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.05% (w/v) Nonidet P-40. 11. WebNov 1, 2010 · A series of protein expression vectors were constructed based on the pEF6/myc-His vector. To generate the pEF6/myc-His vector, a pSecTag2 vector (Invitrogen, USA) was digested with NotI and PmaCI, and the resulting 1.7 kb fragment was inserted into the 4.1 kb NotI – PmaCI digested pEF6/V5-His vector.

Webp3xFLAG-CMV-10 Vector with a neomycin resistance marker for expression of N-terminally 3xFLAG-tagged proteins in mammalian cells. Sequence Author: MilliporeSigma Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Mammalian Expression Vectors More Plasmid Sets WebThe total amount of DNA was adjusted with empty vector pEF6/Myc-His. After 48 h, cell lysates were prepared and immunoprecipitated by anti-FLAG antibody resin. Each sample was analyzed by western blotting with the indicated antibodies. Techniques Used: Immunoprecipitation, Expressing, Transfection, Plasmid Preparation, Western Blot

WebpEF6/myc-His C Description Plasmid type: Mammalian expression vector Copy Number: High copy Promoter: EF-1a Cloning Method: Multiple cloning sites,restriction …

WebAnalyze Sequence: pEF6/myc-His A. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest … four bad boys and me charactersWebBackground: Paraneoplastic pemphigus (PNP) is a malignancy-associated autoimmune disease in which circulating autoantibodies recognize various polypeptides that constitute … four bags of chips cost $6WebOct 29, 2024 · The total amount of DNA was adjusted with empty vector pEF6/Myc-His. After 48 h, cell lysates were prepared and immunoprecipitated by anti-FLAG antibody resin. Each sample was analyzed by western blotting with the indicated antibodies. 3.2. Depletion of DCAF7 or TCP1α reduces the cellular level of ERCC1-XPF four bags fullWebBioVector NTCC Inc. 订购电话TEL:400-800-2947 工作QQ/微信:1843439339 Email:[email protected] discoloration on back whiteWebThe empty vector pEF6/Myc-His A was transfected into the cells to normalize the total amount of DNA per transfection. The cells transfected with the plasmids were infected with FCoV at a multiplicity of infection (MOI) of 1 plaque-forming unit (pfu) per cell, in order to study their effects on FCoV infection 24 h after transfection. discoloration on black skinWebpEF6/myc-His A Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … four bakerlux speed proWebUse pEF6/Myc-HisC for functional genomics research and drug discovery study. 2024-nCoV Research Solution Guide; Monkeypox Virus Real Time PCR Kit; Monkeypox Pseudovirus; … discoloration on heating element dishwasher