WebNov 8, 2024 · The DNA phosphorothioation is physically recognized by the molecular interaction of the sulfur binding domain (SBD) in the Streptomyces coelicolor type IV restriction enzyme ScoM-crA (4, 5). WebMay 12, 2016 · DNA phosphorothioate (PT) modification is a novel physiological variation in a specific sequence and stereo ( i.e ., Rp -PT) manner ( Fig. 1A–C ), in which sulfur is …
RCSB PDB - 7CC9: Sulfur binding domain of SprMcrA complexed …
WebFeb 3, 2010 · First-generation therapeutic AS-ODNs consisted of sulfur-substituted DNA in one of the free oxygen molecules present in the phosphodiester backbone, thus producing PS-ODNs. 136 PS-ODNs resist nuclease degradation while remaining substrates for RNaseH, leading to efficient and specific silencing of the target mRNA in vitro. 137,138 The first PS ... WebDec 23, 2010 · We demonstrated that the S. coelicolor protein Sco4631 is a Type IV REase that cleaves Dcm-methylated and also S-modified (phosphorothioated) DNA in vitro near … headboard fixings b\u0026q
Phosphorothioated DNA Is Shielded from Oxidative Damage
WebFeb 3, 2010 · First-generation therapeutic AS-ODNs consisted of sulfur-substituted DNA in one of the free oxygen molecules present in the phosphodiester backbone, thus producing … WebJul 27, 2015 · A wide variety of prokaryotes possess DNA modifications consisting of sequence-specific phosphorothioates (PT) inserted by members of a five-gene cluster. Recent genome mapping studies revealed... WebJul 5, 2012 · Growth curve experiments showed that S. enterica containing naturally phosphorothioated DNA were more resistant to H 2 O 2 than their mutant derivatives that lack the modification, suggesting that PT modification has anti-oxidative physiological function in the hosting bacteria. It is possible that anti-oxidative function could come … gold hills nursery