WebFIG 5. Effect of rctA on rctB expression. The rctA effect was determined either alone or in conjunction with RctB. (A) A transcriptional rctBp - lacZ fusion as in pDP336 (Fig. 1) was used, but the ... WebDownload scientific diagram Summary of RctB regions important in DNA binding, dimerization and initiation. ( A ) The interval 450–521 of RctB is expanded to show the positions of changes in ...
Effect of rctA on rctB expression. The rctA effect was determined ...
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WebThe rewards include first-in-a-class chemical entities with novel modes of action, as well as a growing microbial seed bank that represents a potentially enormous source of biotechnological and therapeutic innovation. ... The Vibrio cholerae RctB protein is essential for replication initiation at the origin of chromosome II, oriCII. WebDownload scientific diagram Construction of rCTB–YVAD secretion vector pSCTB–YVAD. pSCTB–YVAD includes the promoter of the LDH gene, SS of the β-N-acetylglucosaminidase gene, the CTB ... WebFeb 1, 2013 · Production of rCTB in large scale. BL21 (DE3) competent cells were used as recipient of pAE‐ctxB construct plasmid.Two hundred millilitres of LB broth containing 100 μg ml −1 ampicillin was inoculated with 5 ml of fresh cultures of BL21 (DE3) cells containing pAE‐ctxB plasmid. The cultures were shaken at 180 rev min −1 in 37°C, and isopropyl‐β‐ d … michael hill jewellers chilliwack