WebTHUNDERBIRDTM Next SYBR® qPCR Mix 1 mL THUNDERBIRDTM Next SYBR® qPCR Mix 1.67 mL x 3 . Notes: -THUNDERBIRDTM Next SYBR® qPCR Mix can be stored, protected from light, at 2-8°C for up to 3 months. For longer storage, this reagent should be kept at -20°C and protected from light. No negative effect was detected by 10 freeze-thaw ... WebJun 6, 2024 · The NucleoSpin RNA kit and Thunderbird™ Next SYBR® qPCR mix were purchased from Macherey–Nagel GmbH and Co. KG (Düren, Germany) and Toyobo (Osaka, Japan), respectively. BCA protein assay reagent was obtained from Pierce/Thermo Scientific Inc. (Waltham, MA, USA).

THUNDERBIRD™ SYBR® qPCR Mix - Toyobo - YUMPU

WebMay 11, 2024 · RT-qPCR was performed using a real time PCR system (CFX384; Bio-Rad), and expression levels of genes except for Rtl1 were measured using THUNDERBIRD Next SYBR qPCR Mix (Toyobo) by PCR with an initial denaturation step at 95°C for 30 s followed by 40 cycles of 95°C for 5 s and 60°C for 30 s. WebTHUNDERBIRD™ Next SYBR ® qPCR Mix was used to detect 96 samples using Salmonella genome diluted to one copy as a template. The results showed that 38.5% of the samples were undetected and 61.5% were detected, which is equivalent to the expected number of samples from the Poisson distribution, suggesting that one copy equivalent can be ... how far does amtrak go in florida

THUNDERBIRD TTHUNDERBIRD NextHUNDERBIRD

WebTHUNDERBIRDTM Next SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I. The master mix contains all required components, except … WebTHUNDERBIRD™ SYBR® qPCR Mix 1.67 ml x 3 50xROX reference dye 250 μl x 1 Notes: -THUNDERBIRD™ SYBR® qPCR Mix can be stored, protected from light, at 2-8°C for up to 3 months. For longer storage, this reagent should be kept at -20°C and protected from light. No negative effect was detected by 10 freeze-thaw cycles of hierarchical decision analysis