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Topflash/fopflash reporter

WebDescription. FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three … WebArrowed cells showed nuclear staining of β-catenin. ( G ) Huh7 cells were transfected with equal amounts of either Super8xTOPFlash reporter or Super8xFOPFlash reporter constructs. After 48 hours, the TopFlash/FopFlash luciferase activity of CD133 + and CD133 - Huh7 cells was measured. Values shown are relative luminescence.

TCF Reporter Plasmid Kit - Sigma-Aldrich

WebFOPFlash(报告基因质粒)是TOPFlash(D2501)的阴性对照报告基因质粒。 ... Reporter Vector primer 4 (RVprimer4) binding region: 3537-3556: ColE1-derived plasmid replication origin: 3794: Synthetic Beta-lactamase (Ampr) coding region: 4585-5445: Synthetic poly(A) signal/transcriptional pause site: 5550-5703: TOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame. foot nerve damage tests https://salermoinsuranceagency.com

TOP/FOP-FLASH assay in HEK293T cells? ResearchGate

WebJournal: PLoS ONE Article Title: The natural compound Jatrophone interferes with Wnt/β-catenin signaling and inhibits proliferation and EMT in human triple-negative breast cancer doi: 10.1371/journal.pone.0189864 Article Snippet: The activity of β-catenin dependent Wnt signaling was measured by the TOPFLASH reporter using the Dual Luciferase Reporter … Web14. máj 2024 · TOPFlash/FOPFlash Luciferase Reporter Assays. Wnt-specific TOPFlash/FOPFlash luciferase reporter assays were used to evaluate the effects of these compounds on β-catenin–dependent transcription. WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from … foot nerve damage repair

molecular biology - How does a TOPflash/FOPflash assay work to detect

Category:Assaying β-Catenin/TCF Transcription with β-Catenin/TCF

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Topflash/fopflash reporter

Topflash Reporter Assay Bioz Ratings For Life-Science Research

Web21-169 Sigma-Aldrich FOPflash (mutant TCF binding sites) Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 copies in the reverse orientation. Serves as a negat More>> WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF …

Topflash/fopflash reporter

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WebDescription. FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three … WebThis reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is …

Web14. aug 2015 · zt200 TOPFlash 载体 Millipore 信号通路报告载体系统： zt201 FOPFlash 载体 ... zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 pGreen-puro . zt1230 pGZ . zt1231 pHAGE-CMV-dsRed-UBC-GFP-W ... Web18. jún 2014 · The TOPFlash luciferase reporter gene, which contains 3 copies of a TCF/LEF binding site, and FOPFlash luciferase reporter, identical to TOPFlash except the TCF/LEF sites have been mutated, were purchased from Addgene (Cambridge, MA, USA) [19]. The pRL renilla luciferase vector was from Promega (Madison, WI, USA).

Web4. apr 2024 · Biological and molecular characteristics of GC cell lines and PDOs upon PMCA4 inhibition were investigated using RT-qPCR, western blot, and TOPflash/FOPflash reporter assay. WebKai F. Albring Charité Universitätsmedizin Berlin Hi Jude, monitoring Wnt/beta-catenin activity with Topflash reporters is pretty simple, as long as tranfection is working. There are several...

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WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … foot nerve distribution pictureWebThe FOPflash luciferase reporter plasmid containing mutated TCF/LEF binding sites (mTCF) served as a negative control. Techniques Used: Luciferase, Activity Assay, Cotransfection, … foot nerve distributionWebTOPflash (TCF-Reporterplasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame.; find Sigma-Aldrich-21170 MSDS, related peer-reviewed papers, … foot nerve innervationhttp://www.labbase.net/Supply/SupplyItems-4229347.html foot nerve mapWebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold … foot nerve pain at nightWebTransfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase mi More>> TOPflash (TCF Reporter Plasmid) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS elfed highWebA) DLD-1 cells were transfected with TOPflash or FOPflash reporter vectors and pRL-CMV together with empty vector (CT) or pCI-βcatS37A, the mutated β-catenin expression vector (S37A). The... elfed high school buckley map